Call for tenders' details

Development of a mechanistic model to assess risks to honeybee colonies from exp...
Contracting authority:
European Food Safety Authority (EFSA)
TED publication date:
Time limit for receipt of tenders:
English (en)
Question details
Would our application be eligible? Title: Development of a toolkit to assess toxic interactions between multiple stressors to honeybees.
The basic concept is to identify a range of different fluorescent substrates of cytochrome P450’s and so create a toolkit of probes that together report on the full range of detoxification capacity (honeybee native CYPome. nCYPome) of honeybees (or any other beneficial/pest insect). This will be supported by the production of all honeybee P450 enzyme probes (a honeybee recombinant CYPome, rCYPome) that will allow the molecular basis of the P450 repertoire to be understood. The nCYPome will allow the identification of competing compounds that would become more toxic when encountered together and also to identify particular weaknesses in their detoxification armour. This toolkit will be probed for key stressors to bees to validate the model; key pesticide combinations, Nosema infection, Varroa parasitism and poor diet. The rCYPome will provide a valuable tool to identify the detoxification of new pesticides and could permit a colour-coding system to warn end user of potential incompatibility of multiple pesticides. Importantly, the nCYPome could function as an environmental monitor to indicate locations where honeybees may be under stress. This is particularly relevant as the overall detoxification capacity of honeybees is plastic and may be altered by chronic exposure to particular pesticides, disease or diet. Once validated, similar nCYPomes could be built to inform on the risks to other beneficial insects and for the targeting of pesticide-resistant pests (where their nCYPome may be strengthened by an upregulation of particular P450s) by the use of carefully selected pesticide combinations that circumvent protective P450 changes in expression. Key predictions of interactions between different stressors predicted by the toolkit will be verified by studying pesticide clearance rates in bees and by assessing increased toxicity.
The proposed toolkit of probes reporting on detoxification capacity of honeybees shall prove very useful to predict interactions of key stressors on bees. However, the tender specifications is on the development of a mechanistic model based on the dynamics of the colony and the in-hive products and their interactions with the environment. The proposed toolkit seems to be designed for individual bees and therefore, it is not clear how it would be adapted to the colony in its environment. We therefore invite tenderers to read carefully PART 1 of the tender specifications and if tenderers decide to participate to this call for tenders, they need to refer to Part 2 of the tender specifications where it is indicated how the offer will be evaluated. In addition please refer to Part 3 of the tender specifications where it is indicated how to submit the offer and what should the offer consist of.