Call for tenders' details

Title:
Laboratory analyses of topsoil samples collected in the context of the LUCAS 201...
Contracting authority:
European Commission, Joint Research Centre (JRC)
TED publication date:
31/05/2018
Time limit for receipt of tenders:
02/07/2018
Status:
Closed
Status
20/06/2018
21/06/2018
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Answered
Question details
LOT2:qPCR
Since the proposed requirements for quantification of antibiotic resistance genes are extremely specific, could the high-throughput qPCR of >100,000 reactions be replaced by somewhat more expensive Illumina HiSeq whole-metagenome sequencing given its lower vulnerability to inhibitors, lower detection limit, ability to detect variants with qPCR primer bias, and overall better coverage of all known antibiotic resistance genes?
21/06/2018
As indicated in the Technical Specifications, the use of alternative methods would be acceptable provided that the tenderer can prove that the results would be equivalent to analysis by high-throughput qPCR.